Evaluation Of Anti Obesity Activity Of Citrus Sinensis & Citrus Limon By High Fat Diet Induced Model Using Danio Rerio

Year : 2024 | Volume :14 | Issue : 01 | Page : 24-31

Saswata Mukherjee

Souradeep Datta

Samrat Bose

  1. Postgraduate Student Guru Nanak Institute of Pharmaceutical Science and Technology ,Panihati West Bengal India
  2. Postgraduate Student Guru Nanak Institute of Pharmaceutical Science and Technology ,Panihati West Bengal India
  3. Assistant Professor Guru Nanak Institute of Pharmaceutical Science and Technology, Panihati West Bengal India


Obesity, defined by the World Health Organization (WHO) as an abnormal or excessive fat accumulation that may impair health, is becoming one of the greatest challenges to global health in this millennium, with more than 300 million men and nearly 450 million women obese globally in 2018. Obesity turns into serious problem globally. Zebrafish model can be used extensively for drug screening due to its low maintenance cost, comparatively short period of testing, genetic similarity with human body. Citrus sinensis and Citrus limon contain different pharmacological properties due to its plenty of source of secondary metabolites. Different parts of Citrus sinensis & Citrus limon contain anti-oxidant activity and its contain different pharmacognostic characteristics such as flavonoids, hydroxyamide, steroids, alkanes and alkanes, fatty acids, coumarins, peptides, carbohydrates, carbamates and alkylamines, carotenoids, volatile compounds such as ketones, ester, terpenes, aldehydes, alcohols etc. Fresh and healthy Citrus sinensis & Citrus limon were procured from the locality of Hooghly district, West Bengal in the month of August. A large group of high fat diet induced adult zebra fishes were taken, weighed & divided into control, test (15, 30 and 50 mg/dl concentration), and standard groups. After 42 days, compared the total cholesterol level with control group of fishes (without treated test drug). Citrus sinensis & Citrus limon shows the anti-obesity activity on Danio rerio. Different concentration of test sample (15, 30 and 50 mg/dl) are showing the significant anti-obesity activity on zebra fish model where different standard obesity induced model (by using high fat diet induced) was used. This experimental protocol can be used to continue more comparative studies in future using different dosage form and more variety of citrus fruits, which can lead to more even bigger statistical answer to anti-obesity problems and its natural remedies. Also, this dietary protocol can used to in diabetic model of zebra fishes, to co-relate obesity with diabetes. To finish with, this animal model and experiments were just a very first small step towards a globally emerging problem of obesity and the mental health related to it, there is huge future scope for this type of research work.

Keywords: Anti-obesity, Citrus sinensis, Citrus limon, Danio rerio, Atorvastatin.

[This article belongs to Research & Reviews: A Journal of Pharmacology(rrjop)]

How to cite this article: Saswata Mukherjee, Souradeep Datta, Samrat Bose. Evaluation Of Anti Obesity Activity Of Citrus Sinensis & Citrus Limon By High Fat Diet Induced Model Using Danio Rerio. Research & Reviews: A Journal of Pharmacology. 2024; 14(01):24-31.
How to cite this URL: Saswata Mukherjee, Souradeep Datta, Samrat Bose. Evaluation Of Anti Obesity Activity Of Citrus Sinensis & Citrus Limon By High Fat Diet Induced Model Using Danio Rerio. Research & Reviews: A Journal of Pharmacology. 2024; 14(01):24-31. Available from: https://journals.stmjournals.com/rrjop/article=2024/view=137785


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Regular Issue Subscription Original Research
Volume 14
Issue 01
Received March 11, 2024
Accepted March 21, 2024
Published April 2, 2024