Yong-Mi Ri
Chol-Jin Jang
Yong-Uk Ri
Myong-Song Kim
- Researcher, Pyongyang University of Medical Sciences, Pyongyang, Korea
- Researcher, Pyongyang University of Medical Sciences, Pyongyang, Korea
- Researcher, Pyongyang University of Medical Sciences, Pyongyang, Korea
- Researcher, Pyongyang University of Medical Sciences, Pyongyang, Korea
Abstract
To look at how Gypsophlia (GSP) affects airway inflammation in a model of ovalbumin-induced asthma in guinea pigs (OVA).Methods: Using a random number table, sixty healthy female guinea pigs were split into six groups, each consisting of ten rats: the normal control, OVA, and GSP (0.45, 0.9, 1.8, and 3.6 g/kg). The ovalbumin and lipopolysaccharide sensitization approach was used to create the OVA guinea pig model. The GSP groups received oral GSP (0.9, 1.86g/kg) for 20 days, respectively, at 0.5 h prior to each challenge, whereas the other two groups received an equivalent volume of normal saline. Methacholine aerosol inhalation was done and airway reactivity was assessed 24 hours after the last challenge. The fluid known as bronchoalveolar lavage (BALF) was extracted. For differential counts and total white blood cell counts, Wright-Giemsa staining was employed. Interleukin (IL)-17 and IL-10 levels were measured using an enzyme-linked immunosorbent assay. Results: The airway responsiveness of the OVA group was significantly higher than the normal control group (P<0.05), while those in the GSP groups were significantly lower than the OVA group (P<0.05). The neutrophil and eosinophil counts in the GSP groups were significantly lower than the NA group (P<0.05), and those in the GSP group were significantly lower than the DXM group (P<0.05). There were a large number of peribronchiolar and perivascular inflammatory cells infiltration in the NA group. The airway inflammation in the GSP groups were significantly alleviated than the OVA group. Compared with the normal control group, the IL-17 level in BALF was significantly increased and the IL-10 level in BALF was significantly decreased in the OVA group (P<0.05). GSP treatment significantly decreased IL-17 levels and increased IL-10 levels compared with the OVA group (P<0.05), and the changes in the above indices were more significant in the GSP group (P<0.05). Conclusion: GSP could alleviate the airway inflammation in the OVA –guinea pigs model through increasing the IL-10 level and decreasing the IL-17 level.
Keywords: Gypsophila, Ovalbumin-induced asthma, Airway inflammation, Cytokine, Asthma.
[This article belongs to Research & Reviews: A Journal of Pharmacology(rrjop)]
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Research & Reviews: A Journal of Pharmacology
| Volume | 14 |
| Issue | 01 |
| Received | February 12, 2024 |
| Accepted | March 7, 2024 |
| Published | March 29, 2024 |