Optimization of Seed Viability Tests for Humulus lupulus

Year : 2024 | Volume :13 | Issue : 01 | Page : 1-6

T. Singh

A. Mathur

  1. Research Scholar Department of Biotechnology, Jaypee Institute of Information Technology, Noida Uttar Pradesh India
  2. Associate Professor Department of Biotechnology, Jaypee Institute of Information Technology, Noida Uttar Pradesh India


The Humulus lupulus member of the Hemp family has been extensively explored for their industrial application in diverse sectors. During the cultivation of this high-cost plant, one of the major concerns associated is low seed germination due to physical dormancy. The role of rigid seed coats in augmenting an impermeable barrier for water and nutrient availability to the seed has been well analyzed in previous studies. However, the associated impact on biological dormancy and viability of germplasm has not been explored in detail. Furthermore, in the absence of any well-defined rapid and cost-effective solution, the analysis becomes even more difficult. Thus, there is an urgent need to develop an accurate and reliable, method to check the physiological qualities of the seeds. The present study focuses on the comparative assessment of different staining methods for assessing the viability of Humulus lupulus seeds. The tetrazolium method has been widely used to assess the vigor and viability of various leguminous and horticultural crops. To the best of our literature survey no such methods have been checked to assess the viability of Humulus lupulus seeds. The present study focuses on the qualitative assessment of triphenyl tetrazolium chloride (TTC) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dyes to assess the seed viability. The result revealed that both these dyes can be used to assess the viability of Humulus lupulus seeds however, range of 0.75-1% can be used for evaluating the viability of seeds within an incubation time of 48 hrs.

Keywords: Humulus lupulus, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Seed viability, Surface Morphology, Triphenyl Tetrazolium Chloride (TTC)

[This article belongs to Research & Reviews : Journal of Botany(rrjob)]

How to cite this article: T. Singh, A. Mathur. Optimization of Seed Viability Tests for Humulus lupulus. Research & Reviews : Journal of Botany. 2024; 13(01):1-6.
How to cite this URL: T. Singh, A. Mathur. Optimization of Seed Viability Tests for Humulus lupulus. Research & Reviews : Journal of Botany. 2024; 13(01):1-6. Available from: https://journals.stmjournals.com/rrjob/article=2024/view=143779

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Regular Issue Subscription Original Research
Volume 13
Issue 01
Received March 11, 2024
Accepted April 9, 2024
Published April 19, 2024