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Tripti Singh, Ashwani Mathur
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- Scholar, Associate Professor, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, Uttar Pradesh, India, India
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Abstract
nThe Humulus lupulus member of the Hemp family has been extensively explored for their industrial application in diverse sectors. During the cultivation of this high-cost plant, one of the major concerns associated is low seed germination due to physical dormancy. The role of rigid seed coats in augmenting an impermeable barrier for water and nutrient availability to the seed has been well analyzed in previous studies. However, the associated impact on biological dormancy and viability of germplasm has not been explored in detail. Furthermore, in the absence of any well-defined rapid and cost-effective solution, the analysis becomes even more difficult. Thus, there is an urgent need to develop an accurate and reliable, method to check the physiological qualities of the seeds. The present study focuses on the comparative assessment of different staining methods for assessing the viability of Humulus lupulus seeds. The tetrazolium method has been widely used to assess the vigor and viability of various leguminous and horticultural crops. To the best of our literature survey no such methods have been checked to assess the viability of Humulus lupulus seeds. The present study focuses on the qualitative assessment of triphenyl tetrazolium chloride (TTC) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dyes to assess the seed viability. The result revealed that both these dyes can be used to assess the viability of Humulus lupulus seeds however, range of 0.75-1% can be used for evaluating the viability of seeds within an incubation time of 48 hrs.
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Keywords: Humulus lupulus, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Seed viability, Surface Morphology, Triphenyl Tetrazolium Chloride (TTC)
n[if 424 equals=”Regular Issue”][This article belongs to Research & Reviews : Journal of Botany(rrjob)]
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| Volume | ||
| [if 424 equals=”Regular Issue”]Issue[/if 424][if 424 equals=”Special Issue”]Special Issue[/if 424] [if 424 equals=”Conference”][/if 424] | ||
| Received | March 11, 2024 | |
| Accepted | April 9, 2024 | |
| Published | April 19, 2024 |
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