Evaluation of Inhibition Effect of Nanohybrid Curcumin (Cur-ZnO) and Cur-free Against Some Types of Bacteria

Year : 2023 | Volume : 11 | Issue : 02 | Page : 68-79

    N. Sh. Ahmed

  1. Researcher, College of Agriculture University of Baghdad, Baghdad, Iraq


This study aimed to prepare an efficient nanohybrid food preservative by loading curcumin on zinc oxide. The prepared compound was characterized using microscopy and spectral methods. Fourier transform infrared spectrum of the nanohybrid curcumin showed a shift in the frequencies of some chemical groups towards high and low frequencies. X-ray diffraction (XRS) spectrum also revealed the emergence of new diffraction planes in the nanohybrid compound, Compared with the carrier spectrum of zinc oxide, suggesting that the preservative under study was in the nanoscale. Characterization with atomic force microscope (AFM) confirmed that the average dimeter of the nanohybrid preservative particles was 68.07 nm, while using scanning electron microscope revealed the appearance of zinc oxide in layers and well-defined hexagonal shapes superimposed on top of each other in irregular shape and sizes. Some of these shapes turn into shapes like scattered pieces of wood as well as the formation of compounds. with high porosity in the nanohybrid preservative. The inhibitory efficacy of the nanohybrid curcumin and free curcumin was evaluated against some gram-positive and gram-negative bacterial species isolated from food.

Keywords: Nanohybrid curcumin, characterization, XRD, AFM

This article belongs to Special Issue Conference Material Science and Nanotechnology

How to cite this article: N. Sh. Ahmed Evaluation of Inhibition Effect of Nanohybrid Curcumin (Cur-ZnO) and Cur-free Against Some Types of Bacteria jopc 2023; 11:68-79
How to cite this URL: N. Sh. Ahmed Evaluation of Inhibition Effect of Nanohybrid Curcumin (Cur-ZnO) and Cur-free Against Some Types of Bacteria jopc 2023 {cited 2023 Apr 18};11:68-79. Available from: https://journals.stmjournals.com/jopc/article=2023/view=111756/

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1. Torney F, Trewyn BG, Lin VS, Wang K. Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotechnol. 2007; 2 (5): 295–300.
2. Mirsasaani SS, Hemati M, Dehkord ES, Yazdi GT, Poshtiri DA. Nanotechnology and nanobiomaterials in dentistry. In: Subramnai K, Ahmed W, Harsfield Jr, JK, editors. Nanobiomaterials in Clinical Dentistry. Amsterdam: Elsevier; 2019. pp. 19–37.
3. Abid N, Khan AM, Shujait S, Chaudhary K, Ikram M, Imran M, Haider J, Khan M, Khan Q, Maqbool M. Synthesis of nanomaterials using various top-down and bottom-up approaches, influencing factors, advantages, and disadvantages: a review. Adv Colloid Interface Sci. 2022; 300: 102597.
4. Ahmed NS, Al-Shamary EI. Optimization of phenolic compound production by local Aspergillus niger B1b isolate. IOP Conf Ser: Earth Environ Sci. 2021; 761 (1): 012119.
5. Billes F, Mohammed-Ziegler I, Bombicz P. Vibrational spectroscopic study on the quantum chemical model and the X-ray structure of gallic acid, solvent effect on the structure and spectra. Vibrat Spectrosc. 2007; 43 (1): 193–202.
6. Collee JG, Fraser AG, Marmino BP, Simons A. Mackin and McCartney Practical Medical Microbiology. New York: Churchill Livingstone; 1996.
7. Hinton Jr A, Ingram KD. Use of oleic acid to reduce the population of the bacterial flora of poultry skin. J Food Protect. 2000; 63 (9): 1282–1286.
8. Xie Y, He Y, Irwin PL, Jin T, Shi X. Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Appl Environ Microbiol. 2011; 77 (7): 2325–2331.
9. Katman HY, Khai WJ, Kırgız MS, Nehdi ML, Benjeddou O, Thomas BS, Papatzani S, Rambhad K, Kumbhalkar MA, Karimipour A. Transforming conventional construction binders and grouts into high-performance nanocarbon binders and grouts for today’s constructions. Buildings. 2022; 12 (7): 1041.
10. Sharmin S, Rahaman MM, Sarkar C, Atolani O, Islam MT, Adeyemi OS. Nanoparticles as antimicrobial and antiviral agents: a literature-based perspective study. Heliyon. 2021; 7 (3): e06456.
11. Sirelkhatim A, Mahmud S, Seeni A, Kaus NH, Ann LC, Bakhori SK, Hasan H, Mohamad D. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-micro Lett. 2015; 7: 219–242.
12. Kotha RR, Luthria DL. Curcumin: biological, pharmaceutical, nutraceutical, and analytical aspects. Molecules. 2019; 24 (16): 2930.
13. Wang S, Moustaid-Moussa N, Chen L, Mo H, Shastri A, Su R, Bapat P, Kwun I, Shen CL. Novel insights of dietary polyphenols and obesity. J Nutr Biochem. 2014; 25 (1): 1–8.
14. Pandey A, Chaturvedi M, Mishra S, Kumar P, Somvanshi P, Chaturvedi R. Reductive metabolites of curcumin and their therapeutic effects. Heliyon. 2020; 6 (11): e05469.
15. Panzhinskiy E, Hua Y, Lapchak PA, Topchiy E, Lehmann TE, Ren J, Nair S. Novel curcumin derivative CNB-001 mitigates obesity-associated insulin resistance. J Pharmacol Exp Ther. 2014; 349 (2): 248–257.
16. Teow SY, Liew K, Ali SA, Khoo AS, Peh SC. Antibacterial action of curcumin against Staphylococcus aureus: a brief review. J Trop Med. 2016; 2016: 2853045.
17. Mun SH, Kim SB, Kong R, Choi JG, Kim YC, Shin DW, Kang OH, Kwon DY. Curcumin reverse methicillin resistance in Staphylococcus aureus. Molecules. 2014;19 (11): 18283–18295.
18. Kolekar TV, Yadav HM, Bandgar SS, Deshmukh PY. Synthesis by sol–gel method and characterization of ZnO nanoparticles. Indian Streams Res J. 2011; 1 (1): 1–4.

19. Patil N, Bhaskar RA, Vyavhare VI, Dhadge RA, Khaire VA, Patil YO. Overview on methods of synthesis of nanoparticles. Int J Curr Pharm Res. 2021; 13 (2): 11–16.
20. Fleming I, Williams DH. Spectroscopic Methods in Organic Chemistry. 7th edition. Cham, Switzerland: Springer Nature; 2020. pp. 86–90.
21. Voicu G, Oprea O, Vasile BS, Andronescu E. Antibacterial activity of zinc oxide-gentamicin hybrid material. Dig J Nanomater Biostruct. 2013;8 (3): 1191–1203.
22. Nasir DQ, Wahyuningrum D, Hertadi R. Screening and characterization of levan secreted by halophilic bacterium of Halomonas and Chromohalobacter genuses originated from Bledug Kuwu mud crater. Procedia Chem. 2015; 16: 272–278.
23. Silverstein RM, Bassler GC. Spectrometric identification of organic compounds. J Chem Educ. 1962; 39 (11): 546.
24. Bashi AM, Hussein MZ, Zainal Z, Tichit D. Synthesis and controlled release properties of 2,4-dichlorophenoxy acetate–zinc layered hydroxide nanohybrid. J Solid State Chem. 2013; 203: 19–24.
25. Shafiei S, Birgani ZT, Darvish A, Azimi MS, Solati-Hashjin M. Layered double hydroxides for diagnostic applications. In: International Congress of Evaluation of Medical Diagnosis Modern Technologies 2008. pp. 1–16.
26. Sandıkçı Altunatmaz S, Yılmaz Aksu F, Issa G, Başaran Kahraman B, Dülger Altıner D, Büyükünal SK. Antimicrobial effects of curcumin against L. monocytogenes, S. aureus, S. typhimurium and E. coli O157: H7 pathogens in minced meat. Veterinarni Medicina. 2016; 61 (5): 256–262.
27. Másson M, Holappa J, Hjálmarsdóttir M, Rúnarsson ÖV, Nevalainen T, Järvinen T. Antimicrobial activity of piperazine derivatives of chitosan. Carbohydr Polym. 2008; 74 (3): 566–571.
28. Rai D, Singh JK, Roy N, Panda D. Curcumin inhibits FtsZ assembly: an attractive mechanism for its antibacterial activity. Biochem J. 2008; 410 (1): 147–155.
29. Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. PLoS One. 2015; 10 (3): e0121313.

Conference Open Access Original Research
Volume 11
Issue 02
Received December 8, 2022
Accepted January 31, 2023
Published April 18, 2023