Laser Ablation Technique to Synthesize Ag Nanoparticles in Harsh Environment During Conjugation with Ovalbumin and Their Effects on Candida albicans

Year : 2023 | Volume : 11 | Issue : 02 | Page : 47-60

    Yasmin A. Adi

  1. Student, Al_Iraqia University, College of Medicine, Baghdad, Iraq


In this study, a rapid, easy, eco-friendly and pollution-free-polution method to synthesize Ag nanoparticles (NPs) was developed. Laser ablation technique was used to bulk target silver in harsh environment which was simulated body fluid due to salts and ions content cause Ag NPs to aggregate via the interactions between NPs and components of the ionic solution. Ovalbumin was added to achieve long-term stability of colloidal Ag NPs solution. This protein has been used to decrease ionic strength and avoid nanoparticle agglomeration in biofluids by attaching to the Ag NPs surfaces in the mixture, thereby protecting them from the NaCl and preventing aggregation. The color change on the sample was observed and we examined it with a transmission electron microscope to see its size and
shape, as well as the zeta potential to know its charge, in addition to measuring it in Fourier transform infrared (FTIR) and UV-vis spectrophotometer over a month to prove the stability of the sample. The Ag NPs solution and their dilutions were applied on cultured media of Candida albicans which was isolated from human patients with candida infection.

Keywords: Silver nanoparticles (Ag NPs), pulse laser ablation (PLAL), UV-vis, FTIR, z-potential, TEM, Candida albicans

This article belongs to Special Issue Conference Material Science and Nanotechnology

How to cite this article: Yasmin A. Adi Laser Ablation Technique to Synthesize Ag Nanoparticles in Harsh Environment During Conjugation with Ovalbumin and Their Effects on Candida albicans jopc 2023; 11:47-60
How to cite this URL: Yasmin A. Adi Laser Ablation Technique to Synthesize Ag Nanoparticles in Harsh Environment During Conjugation with Ovalbumin and Their Effects on Candida albicans jopc 2023 {cited 2023 Apr 18};11:47-60. Available from:

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Conference Open Access Original Research
Volume 11
Issue 02
Received December 8, 2022
Accepted January 31, 2023
Published April 18, 2023