Identifying ABO Blood Group and Rh Status Using Dried Saliva Samples

Year : 2024 | Volume : | : | Page : –

Ajit Pal Singh


This article discusses the accuracy of dried saliva samples in establishing ABO and Rh blood types. Participants, likely screened for secretor status beforehand, submitted blood and saliva samples. Both samples were ABO blood typed (A, B, AB, or O) using standard methods such as gel card technology or hemagglutination assays. We used both saliva and blood to perform Rh typing. The findings revealed that saliva and blood typing for ABO groups corresponded nearly perfectly, highlighting the promise of saliva-based ABO identification, particularly for secretors. However, the correlation between the Rh type from saliva and the blood study was significantly weaker. This gap may be due to lower Rh antigen concentrations in saliva or limitations in existing testing techniques. Researchers have demonstrated that saliva serves as a feasible, non-invasive method for identifying the ABO blood group; however, additional research is required to enhance the precision of Rh typing from saliva. Future improvements in this area may include looking into non-invasive alternatives to Rh-factor assessment or developing more sensitive detection methods. A future study should focus on improving the sensitivity of saliva-based Rh detection methods, as well as investigating additional non-invasive methods such as tear or sweat analysis. Future blood group identification will become more comprehensive and reliable with the combination of this and advancements in prenatal Rh factor testing.

Keywords: Saliva-based ABO identification, Rh typing accuracy, Secretor status, Hemagglutination assays, Non-invasive blood typing

How to cite this article: Ajit Pal Singh. Identifying ABO Blood Group and Rh Status Using Dried Saliva Samples. International Journal of Pathogens. 2024; ():-.
How to cite this URL: Ajit Pal Singh. Identifying ABO Blood Group and Rh Status Using Dried Saliva Samples. International Journal of Pathogens. 2024; ():-. Available from:


  1. Yamamoto FI, Clausen H, White T, Marken J, Hakomori SI. Molecular genetic basis of the histo-blood group ABO system. Nature. 1990 May 17;345(6272):229-33.
  2. Allen PZ, Kabat EA. Immunochemical Studies on Blood Groups: XXII. Immunochemical Studies on the Nondialyzable Residue from Partially Hydrolyzed Blood Group A, B and O (H) Substances (P1 Fractions). The Journal of Immunology. 1959 Apr 1;82(4):340-57.
  3. Rydberg L. ABO‐incompatibility in solid organ transplantation. Transfusion Medicine. 2001 Aug;11(4):325-42.
  4. Warner PR, Nester TA. ABO-incompatible solid-organ transplantation. Pathology Patterns Reviews. 2006 Jun 1;125(suppl_1):S87-94.
  5. Bowman JM. The prevention of Rh immunization. Transfusion medicine reviews. 1988 Sep 1;2(3):129-50.
  6. Tripathi R, Singh N. Maternal and perinatal outcome in Rh negative mothers. International Journal of Reproduction, Contraception, Obstetrics and Gynecology. 2018 Aug 1;7(8):3141-7.
  7. Tripathi R, Singh N. Maternal and perinatal outcome in Rh negative mothers. International Journal of Reproduction, Contraception, Obstetrics and Gynecology. 2018 Aug 1;7(8):3141-7.
  8. Wu YY, Csako G. Rapid and/or high-throughput genotyping for human red blood cell, platelet and leukocyte antigens, and forensic applications. Clinica Chimica Acta. 2006 Jan 1;363(1-2):165-76.
  9. Scott ML, Corry JE. Effect of blood group active micro-organisms on the ABO grouping of human whole saliva. Forensic Science International. 1980 Sep 1;16(2):87-100.
  10. Gaensslen RE, Lee HC, Carroll JE. Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping. Zeitschrift für Rechtsmedizin. 1984 Dec;93:259-68.
  11. Miller SM. Saliva testing–a nontraditional diagnostic tool. Clinical laboratory science: journal of the American Society for Medical Technology. 1994 Jan 1;7(1):39-44.
  12. Streckfus CF, Bigler LR. Saliva as a diagnostic fluid. Oral diseases. 2002 Mar;8(2):69-76.
  13. Hofman LF. Human saliva as a diagnostic specimen. The Journal of nutrition. 2001 May 1;131(5):1621S-5S.
  14. Esser D, Alvarez-Llamas G, De Vries MP, Weening D, Vonk RJ, Roelofsen H. Sample stability and protein composition of saliva: implications for its use as a diagnostic fluid. Biomarker insights. 2008 Jan;3:BMI-S607.
  15. Yamamoto F. A historical overview of advances in molecular genetic/genomic studies of the ABO blood group system. Glycoconjugate Journal. 2022 Apr 1:1-2.
  16. Kim CH. Human Red Blood Cell (RBC) Blood Groups System. InGlycoimmunology in Xenotransplantation 2024 Feb 21 (pp. 35-45). Singapore: Springer Nature Singapore.
  17. Smart E, Armstrong B, Reviewer for Second Edition: Edmond Lee. Blood group systems. ISBT Science Series. 2020 Dec;15:123-50.
  18. Jaiswal N, Kumar A. Human secretory and excretory fluids, molecular constituents, and their biotherapeutic perspective against fungal pathogen Candida albicans. Rendiconti Lincei. Scienze Fisiche e Naturali. 2023 Dec;34(4):1105-13.
  19. Pagliaro E. Blood and physiological fluid evidence: Evaluation and initial examination. InIntroduction to Forensic Science and Criminalistics, Second Edition 2019 Jun 20 (pp. 223-253). CRC Press.
  20. Izetbegovic S. Occurrence of ABO and RhD incompatibility with Rh negative mothers. Materia socio-medica. 2013 Dec;25(4):255.
  21. Ajit P, Rahul S, Suyash S. Cytopathology: An Important Aspect of Medical Diagnosis. Research & Reviews: Journal of Oncology and Hematology. 2024 Jan 24;12(03):13-8.
  22. Singh AP, Saxena RA, Saxena SU. A study on the working of blood bank. Journal of Medicine and Health Research. 2022 Jan 10;7(1):1-5.
  23. Bhati A, Varghese A, Rajan G, Sridhar V, Mohan Y, Pradeep S, Babu S, Kaikkolante N, Sarma M, Arun S, Sekar AP. An effective method for saliva stabilization and magnetic nanoparticles based DNA extraction for genomic applications. Analytical Biochemistry. 2021 Jul 1;624:114182.
  24. Knight R. Blood, sweat, and tears: The effort of narrative change in psychoanalysis. The Psychoanalytic Study of the Child. 2008 Jan 1;63(1):292-311.
  25. Van den Veyver IB. Recent advances in prenatal genetic screening and testing. F1000Research. 2016;5.

Ahead of Print Subscription Original Research
Received March 26, 2024
Accepted March 30, 2024
Published May 20, 2024

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