Green Synthesis of Silver Nanoparticles Using Leaf Extract of Calpurnia Aurea and Evaluation of its Antibacterial Activity

Open Access

Year : 2021 | Volume : | Issue : 2 | Page : 25-33

    Birkinesh Girma

  1. Gezahegn Faye

  2. Tilahun Wubalem

  1. Lecturer, Jimma University, Jimma, Ethiopia
  2. Lecturer, Jimma University, Jimma, Ethiopia
  3. PhD, Jimma University, Jimma, Ethiopia


Synthesis of nanoparticles using plant extracts is an emerging as a new and effective method that avoids the involvement of toxic chemicals. In this study AgNPs synthesized using Calpurnia aurea leaf extracts were evaluated for their antibacterial activity. The green synthesized AgNPs were characterized using analytical instruments like UV–visible, X-ray diffraction (XRD) and Fourier transform infrared (FTIR). The UV-Vis spectra shows surface Plasmon resonance peak present at 429 nm. The observed FTIR spectra of the extract exhibited some degree shift in the corresponding nanoparticles, this result clearly showed that the extracts contain functional groups act in capping the nanoparticles. XRD analysis revealed that silver nanoparticle was crystalline in nature and has facecentered cubic geometry. The antibacterial activity of both AgNPs and extract were evaluated against Bacillus subtilis and Escherichia coli. Bacteria using agar well diffusion assay. The synthesized AgNPs showed enhanced antibacterial potential against Bacillus subtilis than Escherichia coli.

Keywords: Antibacterial, Silver nanoparticles, Bacillus subtilis, Escherichia coli, Calpurnia aurea

[This article belongs to International Journal of Nanomaterials and Nanostructures(ijnn)]

How to cite this article: Birkinesh Girma, Gezahegn Faye, Tilahun Wubalem Green Synthesis of Silver Nanoparticles Using Leaf Extract of Calpurnia Aurea and Evaluation of its Antibacterial Activity ijnn 2021; 7:25-33
How to cite this URL: Birkinesh Girma, Gezahegn Faye, Tilahun Wubalem Green Synthesis of Silver Nanoparticles Using Leaf Extract of Calpurnia Aurea and Evaluation of its Antibacterial Activity ijnn 2021 {cited 2021 Dec 24};7:25-33. Available from:

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Regular Issue Open Access Article
Volume 7
Issue 2
Received November 25, 2021
Accepted December 14, 2021
Published December 24, 2021