Recovery of mycelial biomass of Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus: A comparative study

Year : 2024 | Volume : | : | Page : –
By

Apeksha Rathi

Prof. Neeraj Wadhwa

  1. Research Scholar Department of Biotechnology, Jaypee Institute of Information Technology, A-10 Sector-62, Noida Uttar Pradesh India
  2. Professor Department of Biotechnology, Jaypee Institute of Information Technology, A-10 Sector-62, Noida Uttar Pradesh India

Abstract

Mushrooms are the spore-bearing fruiting body of fungi which has a significant potential for numerous applications like pharmaceuticals, textile industry, bioremediation, soil management, alternative to toxic packaging materials and environment sustainability. Mushrooms generally belongs to the class Basidiomycota or Ascomycota. They are typically made of hyphae, the tiniest threads which comprises most fungi. Mycelia are tiny thread like structure which help in making vegetative part of fungus. In this study, the growth of mycelia from four edibles mushroom species Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus in Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) was compared. For identification of mycelia lactophenol cotton blue staining was performed. Interestingly, there was a noticeable difference in the mycelial biomass yield between (PDA) and (PDB). We report morphological characteristics of the mycelia on the basis of colour, texture, growth time requirement. There was a significant difference in yield of mycelial biomass in the (PDA) and (PDB). We could recover 1.760 gm of Pleurotus ostreatus mycelia, of 0.260 gm Agaricus Bisporus mycelia, 0.038gm of Ganoderma lucidum and 0.427 gm Hieracium Erinaceus mycelial biomass after 15 days of culturing on (PDA) agar plates. Recovery of mycelial biomass on (PDA) was better than (PDB). One of the effective methods for producing fungal biomass is to cultivate it on PDA. Our findings showed that the two media differed significantly in the mycelial biomass output, with (PDA) generally outperforming (PDB). These findings underscore the importance of cultivation medium selection in maximizing mycelial biomass production. The utilization of fungal biomass can prove to be helpful in different areas such as medicine, nutrition, agriculture, and sustainability of environment

Keywords: Mycelial growth, PDA, PDB, Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus

How to cite this article: Apeksha Rathi, Prof. Neeraj Wadhwa. Recovery of mycelial biomass of Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus: A comparative study. Research & Reviews: A Journal of Microbiology & Virology. 2024; ():-.
How to cite this URL: Apeksha Rathi, Prof. Neeraj Wadhwa. Recovery of mycelial biomass of Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus: A comparative study. Research & Reviews: A Journal of Microbiology & Virology. 2024; ():-. Available from: https://journals.stmjournals.com/rrjomv/article=2024/view=0


References

  1. Nagulwar M, More D, Mandhare L. Nutritional properties and value addition of mushroom: A review. Pharma Innov J. 2020;9:395-8.
  2. Dermiki M, Phanphensophon N, Mottram DS, Methven L. Contributions of non-volatile and volatile compounds to the umami taste and overall flavour of shiitake mushroom extracts and their application as flavour enhancers in cooked minced meat. Food chemistry. 2013;141(1):77-83.
  3. Cheung PC. The nutritional and health benefits of mushrooms. Nutrition Bulletin. 2010;35(4):292-9.
  4. Li H, Tian Y, Menolli Jr N, Ye L, Karunarathna SC, Perez‐Moreno J, et al. Reviewing the world’s edible mushroom species: A new evidence‐based classification system. Comprehensive Reviews in Food Science and Food Safety. 2021;20(2):1982-2014.
  5. Kumar K, Mehra R, Guiné RP, Lima MJ, Kumar N, Kaushik R, et al. Edible Mushrooms: A comprehensive review on bioactive compounds with health benefits and processing aspects. Foods. 2021;10(12):2996.
  6. Haneef M, Ceseracciu L, Canale C, Bayer IS, Heredia-Guerrero JA, Athanassiou A. Advanced materials from fungal mycelium: fabrication and tuning of physical properties. Scientific reports. 2017;7(1):41292.
  7. Jones M, Huynh T, Dekiwadia C, Daver F, John S. Mycelium composites: a review of engineering characteristics and growth kinetics. Journal of Bionanoscience. 2017;11(4):241-57.
  8. Argyropoulos D, Psallida C, Sitareniou P, Flemetakis E, Diamantopoulou P. Biochemical evaluation of Agaricus and Pleurotus strains in batch cultures for production optimization of valuable metabolites. Microorganisms. 2022;10(5):964.
  9. Harley JL, Waid JS. A method of studying active mycelia on living roots and other surfaces in the soil. Transactions of the British Mycological Society. 1955;38(2):104-18.
  10. Fricker MD, Heaton LL, Jones NS, Boddy L. The mycelium as a network. The fungal kingdom. 2017:335-67.
  11. Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annual Reviews in Microbiology. 1968;22(1):87-108.
  12. Kim S, Hwang H, Park J, Cho Y, Song C, Yun J. Mycelial growth and exo‐biopolymer production by submerged culture of various edible mushrooms under different media. Letters in Applied Microbiology. 2002;34(1):56-61.
  13. Kandasamy S, Chinnappan S, Thangaswamy S, Balakrishnan S, Khalifa AY. Assessment of antioxidant, antibacterial activities and bioactive compounds of the wild edible mushroom Pleurotus sajor-caju. International Journal of Peptide Research and Therapeutics. 2020;26:1575-81.
  14. Friedman M. Chemistry, nutrition, and health-promoting properties of Hericium erinaceus (Lion’s Mane) mushroom fruiting bodies and mycelia and their bioactive compounds. Journal of Agricultural and Food Chemistry. 2015;63(32):7108-23.
  15. Souilem F, Fernandes Â, Calhelha RC, Barreira JC, Barros L, Skhiri F, et al. Wild mushrooms and their mycelia as sources of bioactive compounds: Antioxidant, anti-inflammatory and cytotoxic properties. Food chemistry. 2017;23 0:40-8.
  16. Dong CH, Yao YJ. Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture. Journal of Applied Microbiology. 2005;99(3):483-92.
  17. Jones M, Mautner A, Luenco S, Bismarck A, John S. Engineered mycelium composite construction materials from fungal biorefineries: A critical review. Materials & Design. 2020;187:108397.
  18. Houette T, Maurer C, Niewiarowski R, Gruber P. Growth and mechanical characterization of mycelium-based composites towards future bioremediation and food production in the material manufacturing cycle. Biomimetics. 2022;7(3):103.
  19. Lange J, Wyser Y. Recent innovations in barrier technologies for plastic packaging—a review. Packaging Technology and Science: An International Journal. 2003;16(4):149-58.
  20. Ly L, Jitjak W, editors. Formation of 3-dimensional bio-composite from local mushroom mycelia. The 5th TICC International Conference; 2020.
  21. Bustillos J, Loganathan A, Agrawal R, Gonzalez BA, Perez MG, Ramaswamy S, et al. Uncovering the mechanical, thermal, and chemical characteristics of biodegradable mushroom leather with intrinsic antifungal and antibacterial properties. ACS Applied Bio Materials. 2020;3(5):3145-56.
  22. Krupodorova TA, Barshteyn VY. Alternative substrates for higher mushrooms mycelia cultivation. Journal of BioScience & Biotechnology. 2015;4(3).
  23. Badalyan SM, Zambonelli A. Biotechnological exploitation of macrofungi for the production of food, pharmaceuticals and cosmeceuticals. Advances in macrofungi: diversity, ecology and biotechnology. 2019:199-230.
  24. Karatay SE, Dönmez G. Evaluation of biotechnological potentials of some industrial fungi in economical lipid accumulation and biofuel production as a field of use. Preparative Biochemistry and Biotechnology. 2014;44(4):332-41.
  25. Gujre N, Soni A, Rangan L, Tsang DC, Mitra S. Sustainable improvement of soil health utilizing biochar and arbuscular mycorrhizal fungi: A review. Environmental Pollution. 2021;268:115549.
  26. Tudses N. Isolation and mycelial growth of mushrooms on different yam-based culture media. Journal of Applied Biology and Biotechnology. 2016;4(5):033-6.
  27. Dar B, Kyu MT, Nyunt KS, Myint NS, Soe KT, Aye WY, et al. Preparation of Fungal and Bacterial Spores’ formation Slides: MERAL Portal; 2015.
  28. Sardar H, Ali MA, Ayyub CM, Ahmed R. Effects of different culture media, temperature and pH levels on the growth of wild and exotic Pleurotus species. Pakistan Journal of Phytopathology. 2015;27(2):139-45.

Ahead of Print Subscription Review Article
Volume
Received April 19, 2024
Accepted May 28, 2024
Published July 3, 2024

function myFunction2() {
var x = document.getElementById(“browsefigure”);
if (x.style.display === “block”) {
x.style.display = “none”;
}
else { x.style.display = “Block”; }
}
document.querySelector(“.prevBtn”).addEventListener(“click”, () => {
changeSlides(-1);
});
document.querySelector(“.nextBtn”).addEventListener(“click”, () => {
changeSlides(1);
});
var slideIndex = 1;
showSlides(slideIndex);
function changeSlides(n) {
showSlides((slideIndex += n));
}
function currentSlide(n) {
showSlides((slideIndex = n));
}
function showSlides(n) {
var i;
var slides = document.getElementsByClassName(“Slide”);
var dots = document.getElementsByClassName(“Navdot”);
if (n > slides.length) { slideIndex = 1; }
if (n (item.style.display = “none”));
Array.from(dots).forEach(
item => (item.className = item.className.replace(” selected”, “”))
);
slides[slideIndex – 1].style.display = “block”;
dots[slideIndex – 1].className += ” selected”;
}