[{“box”:0,”content”:”[if 992 equals=”Open Access”]n
n
Open Access
nn
n
n[/if 992]n
n
n
n
n
n
Eshaana Janardhan Raichur
n
- n t
n
n
n[/foreach]
n
n[if 2099 not_equal=”Yes”]n
- [foreach 286] [if 1175 not_equal=””]n t
- Research Intern Department of Bioinfomatics, BioNome, Bnagalore Karnataka India
n[/if 1175][/foreach]
n[/if 2099][if 2099 equals=”Yes”][/if 2099]n
Abstract
nObjectives: Human papillomavirus (HPV), especially high-risk variants like type-16 have been linked to the formation of malignant tumors. HPV-16 has been implicated in most cases of cervical cancer in women. This study aims to assess the effectiveness of three types of temporin molecules, i.e, antiviral peptides secreted by certain frog species, for the inhibition of the viral L1 protein.
Methods: In the present study, various computational tools were used to find the temporin molecule that was most effective in inhibiting the attachment of the L1 viral major capsid protein of HPV type-16 to the heparan sulfate proteoglycan residues present on the basement membrane of the host cell. HPEPDOCK 2.0 and pyDockWEB servers were used to carry out protein-peptide and protein-protein docking, respectively. Binding affinity scores revealed the efficiencies of the different temporin molecules in inhibiting the action of the L1 protein.
Results:According to the results obtained from this docking study, the temporin-L peptide, which was isolated from Rana temporarina was found to be the most efficient in preventing the attachment of the viral L1 major capsid protein to the host cell HSPG residues present on the basement membrane, thereby hindering the mechanism of viral entry.
Conclusion: Further studies can be conducted on the mechanism of action of the temporin-L antiviral peptide. It can be a potential new therapeutic option for the treatment and management of high-risk HPV infections. Further research can be conducted on the temporin-L molecule for the formulation of new antiviral therapeutics for the treatment of HPV-16 infections. Temporins can be formulated into topical creams or gels, which could be applied directly to genital warts caused by HPV. These formulations might help reduce the size and discomfort of warts or even promote their clearance.
n
Keywords: Human papillomavirus, temporins, antiviral peptides, L1 protein
n[if 424 equals=”Regular Issue”][This article belongs to International Journal of Cell Biology and Cellular Functions(ijcbcf)]
n
n
n
n
n
nn
nn[if 992 equals=”Open Access”] Full Text PDF Download[/if 992] n[if 992 not_equal=”Open Access”]
[/if 992]n[if 992 not_equal=”Open Access”] nnn[/if 992]nn[if 379 not_equal=””]n
Browse Figures
n
n[/if 379]n
References
n[if 1104 equals=””]n
- Dhanuthai K., Rojanawatsirivej S., Thosaporn W., Kintarak S., Subarnbhesaj A., Darling M., Kryshtalskyj E., Chiang C.P., Shin H.I., Choi S.Y., et al. Oral cancer: A multicenter study. Med. Oral Patol. Oral Cir. Bucal. 2018;23:e23–e29.
- International Agency for Research on Cancer.; Available online: http://gco.iarc.fr/
- Cancer Registry Annual Report, 2016 (Taiwan); Available online: https://www.hpa.gov.tw/Pages/Detail.aspx?nodeid=269&pid=12235
- Chuang S.L., Su W.W., Chen S.L., Yen A.M., Wang C.P., Fann J.C., Chiu S.Y., Lee Y.C., Chiu H.M., Chang D.C., et al. Population-based screening program for reducing oral cancer mortality in 2,334,299 Taiwanese cigarette smokers and/or betel quid chewers. Cancer. 2017; 123:1597–1609.
- Pakshir P., Hinz B. The big five in fibrosis: Macrophages, myofibroblasts, matrix, mechanics, and miscommunication. Matrix Biol. 2018;68–69:81–93.
- Hinz B., Pittet P., Smith-Clerc J., Chaponnier C., Meister J.J. Myofibroblast development is characterized by specific cell-cell adherens junctions. Mol. Biol. Cell. 2004;15:4310–4320.
- Wrobel L.K., Fray T.R., Molloy J.E., Adams J.J., Armitage M.P., Sparrow J.C. Contractility of single human dermal myofibroblasts and fibroblasts. Cell Motil. Cytoskelet. 2002;52:82–90.
- Hinz B., Dugina V., Ballestrem C., Wehrle-Haller B., Chaponnier C. Alpha-smooth muscle actin is crucial for focal adhesion maturation in myofibroblasts. Mol. Biol. Cell. 2003;14:2508–2519.
- Hinz B., Celetta G., Tomasek J.J., Gabbiani G., Chaponnier C. Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol. Biol. Cell. 2001;12:2730–2741.
- Etemad-Moghadam S, Khalili M, Tirgary F, Alaeddini M. Evaluation of myofibroblasts in oral epithelial dysplasia and squamous cell carcinoma. J Oral Pathol Med. 2009;38:639–43.
- Thode C, Jørgensen TG, Dabelsteen E, Mackenzie I, Dabelsteen S. Significance of myofibroblasts in oral squamous cell carcinoma. J Oral Pathol Med. 2011;40:201–7.
- Angadi PV, Kale AD, Hallikerimath S. Evaluation of myofibroblasts in oral submucous fibrosis: Correlation with disease severity. J Oral Pathol Med. 2011;40:208–13.
- Vered M, Dobriyan A, Dayan D, Yahalom R, Talmi YP, Bedrin L, et al. Tumor-host histopathologic variables, stromal myofibroblasts and risk score, are significantly associated with recurrent disease in tongue cancer. Cancer Sci. 2010;101:274–80.
- Prasad BV, Kakatkar GS, Jain P, Jain M, Patel M, Khan J. Expression of Myofibroblasts in Oral Squamous Cell Carcinoma: An Immunohistochemical Study. J Contemp Dent Pract. 2016 Oct 1;17(10):857–860.
- Gandhi P, Kaur M, Punia RS, Halappa TS, Singh HP. Myofibroblasts as important diagnostic and prognostic indicators of oral squamous cell carcinoma: An immunohistochemical study using alpha-smooth muscle actin antibody. Journal of Oral and Maxillofacial Pathology: JOMFP. 2022 Apr;26(2):156.
nn[/if 1104][if 1104 not_equal=””]n
- [foreach 1102]n t
- [if 1106 equals=””], [/if 1106][if 1106 not_equal=””],[/if 1106]
n[/foreach]
n[/if 1104]
nn
nn[if 1114 equals=”Yes”]n
n[/if 1114]
n
n
n
International Journal of Cell Biology and Cellular Functions
n
n
n
n
n
n
| Volume | 14 | |
| [if 424 equals=”Regular Issue”]Issue[/if 424][if 424 equals=”Special Issue”]Special Issue[/if 424] [if 424 equals=”Conference”][/if 424] | 01 | |
| Received | April 18, 2024 | |
| Accepted | May 13, 2024 | |
| Published | May 24, 2024 |
n
n
n
n
n
n function myFunction2() {n var x = document.getElementById(“browsefigure”);n if (x.style.display === “block”) {n x.style.display = “none”;n }n else { x.style.display = “Block”; }n }n document.querySelector(“.prevBtn”).addEventListener(“click”, () => {n changeSlides(-1);n });n document.querySelector(“.nextBtn”).addEventListener(“click”, () => {n changeSlides(1);n });n var slideIndex = 1;n showSlides(slideIndex);n function changeSlides(n) {n showSlides((slideIndex += n));n }n function currentSlide(n) {n showSlides((slideIndex = n));n }n function showSlides(n) {n var i;n var slides = document.getElementsByClassName(“Slide”);n var dots = document.getElementsByClassName(“Navdot”);n if (n > slides.length) { slideIndex = 1; }n if (n (item.style.display = “none”));n Array.from(dots).forEach(n item => (item.className = item.className.replace(” selected”, “”))n );n slides[slideIndex – 1].style.display = “block”;n dots[slideIndex – 1].className += ” selected”;n }n”}]