Open Access   Special Issue    Topic

PCR in COVID-19 testing and Cycle threshold values

  • Special Issue Description
  • Keywords
  • Manuscript Submission information
  • Published Articles
  •   This special issue belongs to

      International Journal of Molecular Biotechnology

      Related section


      Deadline for Manuscript Submission

      March 31st, 2022

      Deadline for Publication

      April 15, 2022

    Special Issue Description

    Dear Collegues,

    A modified form of PCR known as the quantitative polymerase chain reaction is used for COVID-19 testing (qPCR). To assess the amount of genetic material in a sample, this technique incorporates fluorescent dyes into the PCR process. Here, healthcare professionals count the genetic material from SARS-CoV-2. Reverse transcription, which transforms the PCR from single-stranded RNA to double-stranded DNA, comes first. Then, the two strands of the DNA template are split apart. One of the most used laboratory techniques for detecting the COVID-19 virus is real-time RT-PCR.

    Although many nations have employed real-time RT-PCR to diagnose other illnesses like the Ebola and Zika viruses, many still want assistance in adapting this technique for the COVID-19 virus and expanding their national testing capacities. A real-time PCR assay detects a positive reaction by building up a fluorescent signal. The number of cycles needed for the fluorescent signal to cross the threshold is known as the Ct (cycle threshold) (i.e., Exceeds the background level). The amount of target nucleic acid in the sample is inversely correlated with the Ct levels, meaning that the lower the Ct level, the higher the amount of target nucleic acid in the sample.


    * PCR
    * qPCR
    * RT-PCR
    * Ct Values
    * COVID-19
    * SARS-CoV2
    * DNA
    * Zika
    * Ebola

    Manuscript Submission information

    Manuscripts should be submitted online by registering and logging in to this link. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed.
    Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent on email address:[email protected] for announcement on this website.
    Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page.

    Published articles

    "This special issue is now open for submission."