PCR in COVID-19 testing and Cycle threshold values
This special issue belongs to
|International Journal of Molecular Biotechnology
Deadline for Manuscript Submission
|March 31st, 2022
Deadline for Publication
|April 15, 2022
Special Issue Description
A modified form of PCR known as the quantitative polymerase chain reaction is used for COVID-19 testing (qPCR). To assess the amount of genetic material in a sample, this technique incorporates fluorescent dyes into the PCR process. Here, healthcare professionals count the genetic material from SARS-CoV-2. Reverse transcription, which transforms the PCR from single-stranded RNA to double-stranded DNA, comes first. Then, the two strands of the DNA template are split apart. One of the most used laboratory techniques for detecting the COVID-19 virus is real-time RT-PCR.
Although many nations have employed real-time RT-PCR to diagnose other illnesses like the Ebola and Zika viruses, many still want assistance in adapting this technique for the COVID-19 virus and expanding their national testing capacities. A real-time PCR assay detects a positive reaction by building up a fluorescent signal. The number of cycles needed for the fluorescent signal to cross the threshold is known as the Ct (cycle threshold) (i.e., Exceeds the background level). The amount of target nucleic acid in the sample is inversely correlated with the Ct levels, meaning that the lower the Ct level, the higher the amount of target nucleic acid in the sample.
* Ct Values
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