Open Access   Special Issue    Topic

Mechanics of Molecules and Thermodynamics analysis of plasmid DNA

  • Special Issue Description
  • Keywords
  • Manuscript Submission information
  • Published Articles
  •   This special issue belongs to

      International Journal of Biochemistry and Biomolecules

      Related section

       NA

      Deadline for Manuscript Submission

      March 31st, 2022

      Deadline for Publication

      April 15, 2022

    Special Issue Description


    Dear Collegues,



    Isothermal titration calorimetry (ITC), dynamic light scattering, surface tension measurements, and UV spectroscopy were used to examine the chemical mechanism and thermodynamics of the interactions between plasmid DNA and cationic surfactants. The cationic surfactants investigated include cetylpyridinium chloride, benzyldimethyldodecylammonium chloride, and cetyl dimethyl tetradecyl ammonium chloride. The findings point to a critical aggregation concentration (CAC) of a surfactant, below which there is no discernible interaction between DNA and surfactant. Above the CAC, the surfactant clumps with plasmid DNA.

    Longer hydrocarbon chains in surfactants result in smaller CAC, suggesting that hydrophobic interaction is important for DNA-surfactant complexation. Additionally, an increase in ionic strength (I) raises the critical micellization concentration (CMC) while lowering the CAC (CMC). Due to these opposing effects, a critical ionic strength (I(c)) is reached at CAC = CMC; if I(c) is lower, CAC decreases; if I(c) is higher, DNA does not form complexes with surfactant micelles. Since the CAC is constant throughout a large range of DNA concentrations, DNA in the interaction displays a pseudo-phase feature. The ITC data demonstrated that the reaction is exclusively driven by entropy because both delta H(o) and deltaS(o) have positive values, ranging from roughly 70 to 110 J K(-1) mol(-1). The combination has a DNA phosphate to surfactant molar ratio of 0.63 to 0.05. Submicrometer-sized primary particles are produced by the process, which agglomerates at high surfactant concentrations.

    Keywords

    * Isothermal titration calorimetry
    * DNA
    * Critical micellization concentration
    * DNA phosphate
    * Plasmid DNA

    Manuscript Submission information


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    Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page.

    Published articles

    "This special issue is now open for submission."