International Journal of Molecular Biotechnological Research

Editors Overview

ijmbr maintains an Editorial Board of practicing researchers from around the world, to ensure manuscripts are handled by editors who are experts in the field of study.

Open Access
Special Issue

PCR in COVID-19 testing and Cycle threshold values

Abstract Submission Deadline : November 30, 2023

Manuscript Submission Deadline : December 25, 2023

Special Issue Description

A modified form of PCR known as the quantitative polymerase chain reaction is used for COVID-19 testing (qPCR). To assess the amount of genetic material in a sample, this technique incorporates fluorescent dyes into the PCR process. Here, healthcare professionals count the genetic material from SARS-CoV-2. Reverse transcription, which transforms the PCR from single-stranded RNA to double-stranded DNA, comes first. Then, the two strands of the DNA template are split apart. One of the most used laboratory techniques for detecting the COVID-19 virus is real-time RT-PCR. Although many nations have employed real-time RT-PCR to diagnose other illnesses like the Ebola and Zika viruses, many still want assistance in adapting this technique for the COVID-19 virus and expanding their national testing capacities. A real-time PCR assay detects a positive reaction by building up a fluorescent signal. The number of cycles needed for the fluorescent signal to cross the threshold is known as the Ct (cycle threshold) (i.e., Exceeds the background level). The amount of target nucleic acid in the sample is inversely correlated with the Ct levels, meaning that the lower the Ct level, the higher the amount of target nucleic acid in the sample.


PCR, qPCR, RT-PCR, Ct Values, COVID-19, SARS-CoV2, DNA, Zika, Ebola

Manuscript Submission information

Manuscripts should be submitted online via the manuscript Engine. Once you register on APID, click here to go to the submission form. Manuscripts can be submitted until the deadline.
All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the email address:[email protected] for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a Double-blind peer-review process. A guide for authors and other relevant information for the submission of manuscripts is available on the Instructions for Authors page.

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