Akshita Gupta,
Rekha Karwasra,
- Research Scholar Department of Basics and Applied Sciences, Nirwan University Jaipur Rajasthan India
- Professor Department of Basics and Applied Sciences, Nirwan University Jaipur Rajasthan India
Abstract
This study employs Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry to analyze MRSA peptides, focusing on various parameters associated with mass-to-charge (m/z) values. Through systematic data collection and analysis, including time, intensity, signal-to-noise ratios (SN), quality factors, resolutions, areas under the peaks, relative intensities, full widths at half maximum (FWHM), chi-squared values, and background peaks, comprehensive insights into the spectral characteristics of MRSA peptides are obtained. Methicillin-resistant Staphylococcus aureus (MRSA) is a major public health concern due to its resistance to commonly used antibiotics and its ability to cause severe infections. This study provides a comprehensive analysis of MRSA peptides using Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry to identify potential biomarkers and therapeutic targets. MRSA isolates were collected from clinical samples obtained from patients in a tertiary care hospital over a six-month period. The isolates were cultured and subjected to protein extraction procedures. Peptide profiles were generated using MALDI mass spectrometry, and the resulting spectra were analyzed to identify specific peptides associated with MRSA. The analysis revealed a distinct peptide signature unique to MRSA isolates, differentiating them from methicillin-sensitive Staphylococcus aureus (MSSA) and other bacterial strains. Several peptides were identified as potential biomarkers for MRSA, including those involved in antibiotic resistance, virulence, and biofilm formation. Notably, peptides related to the mecA gene, responsible for methicillin resistance, were consistently detected in MRSA isolates. Additionally, comparative analysis of peptide profiles from different MRSA strains highlighted variations in protein expression related to geographic and clinical sources, providing insights into the epidemiology and evolution of MRSA.
Keywords: MRSA, peptides, MALDI-TOF, mass spectrometry, antibiotic resistance.
[This article belongs to Research & Reviews: A Journal of Pharmaceutical Science(rrjops)]
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Research & Reviews: A Journal of Pharmaceutical Science
| Volume | 15 |
| Issue | 02 |
| Received | June 6, 2024 |
| Accepted | July 3, 2024 |
| Published | July 16, 2024 |