Ali S. Shakir,
Alaa T. Hamza,
- Lecturer Department of Clinical Immunology College of Dentistry, University of Al-Qadisiyah, Diwaniya Iraq
- Lecturer Department of Biochemistry, College of Dentistry, University of Al- Qadisiyah Diwaniya Iraq
Abstract
In order for vitamin D to have an impact, the vitamin D receptor (VDR) must be expressed and activated in the nucleus. The VDR has a number of known genetic variants. Biological impacts can be caused by changes in DNA sequences known as “polymorphisms” that are common in the population. Vitiligo is a disease that causes loss of skin color in patches, and it is a chronic (long-lasting) autoimmune disorder loss of color. The skin turns a milky white color because the skin cells that create pigment, called melanocytes, are attacked and destroyed. For comparison, the study included 80 samples total; 40 of them came from vitiligo sufferers (male and female) and 40 from healthy individuals. Under aseptic conditions, five milliliters of blood were collected by puncturing a vein with disposable syringes. The 2 ml of each sample was placed in an EDTA tube and kept at -20 C until the polymerase chain reaction amplification and detection of the vitamin D binding protein BP (rs2282679) gene (ARMS-PCR) method could be performed in order to avoid repetitive thawing and freezing. The remaining 3 ml were moved to a sterile gel tube, left to clot at room temperature, and then spun at 2500 rpm for 10 minutes to prevent the Vitamin D ELISA Kit (Mabtech USA) from undergoing repeated thawing and freezing. The serum that had been isolated was placed in Eppendorf tubes and quickly refrigerated at -20 C until it could be used again. With p=0.644 for gender and p=0.813 for age, the results did not show any significant differences between the groups. The current study showed (37.5%) of Vitiligo patients have a positive family history, 25(OH) D level decreased in inpatients compared to control, and showed that The most abundant gene is AA in vitiligo patients compared to controls, and this enhanced decreased result of 25(OH) D. Also suggested an association between Vitamin-D binding-protein BP rs2282679 polymorphism and the levels of Vitamin-D in Vitiligo patient.
Keywords: Vitiligo, Polymorphism, Vitamin D, Genotype, Cholecalciferol
[This article belongs to Research & Reviews : A Journal of Biotechnology(rrjobt)]
References
- Mazzei Weiss M. E., (2020). Vitiligo: to biopsy or not to biopsy? Cutis. 105(4):189-190.
- Gandhi K., Ezzedine K., Anastassopoulos K.P., Patel R., Sikirica V., et al., (2022). Prevalence of Vitiligo Among Adults in the United States. JAMA Dermatol. 158(1):43-50.
- Bouillon P., Manousaki D., Rosen C., Trajanoska K., Rivadeneira F.& Richards J.B. (2022).The health effects of vitamin D supplementation: evidence from human studies. Nature Reviews Endocrinology volume.18:96–110.
- El-Sharkawy A. and Malki A. (2020). Vitamin D Signaling in Inflammation and Cancer: Molecular Mechanisms and Therapeutic Implications. Molecules.25(14), 3219
- Ravaioli F., Pivetti A., Marco L.D., Chrysanthi C., Frassanito G., Pambianco M., Sicuro C., Gualandi N., Guasconi T., Pecchini M. and Colecchia A.(2022). Role of Vitamin D in Liver Disease and Complications of Advanced Chronic Liver Disease. Int. J. Mol. Sci. 23(16): 9016.
- Varghese J.E., Balasubramanian B.,Velayuthaprabhu S., Thirunavukkarasu V., Rengarajan R.L., Murugesh E., Manikandan P., Arun M. and Anand A.V. (2021). Therapeutic effects of vitamin D and cancer: An overview. Food Frontiers. 2(4):1–9.
- Murray S.L., Wolf M. (2023). Calcium and Phosphate Disorders: Core Curriculum 2024. CORE CURRICULUM IN NEPHROLOGY.(83)2:241-256.
- Zafalon R.V.A., Ruberti B., Rentas M.F., Amaral A.R., Vendramini T.H.A., Chacar F.C., Kogika M.M.,2 and Brunetto M.A. (2020). The Role of Vitamin D in Small Animal Bone Metabolism. Metabolites.10(12): 496.
- Asghari G., Yuzbashian E., Najd-Hassan-Bonab L., Mirmiran P., Daneshpour M.S.,corresponding and Azizi F.(2023). Association of rs2282679 polymorphism in vitamin D binding protein gene (GC) with the risk of vitamin D deficiency in an iranian population: season-specific vitamin D status. BMC Endocr Disord.23: 217.
- Katsarou1 M., Sidiropoulou P., Ieronymaki D., Mastraftsi S., Sifaki M., Xenos K., Nosyrev A., Kovatsi L., Spandidos D., Lagiou M., Dagklis C., Gregoriou S., Tagka A., Rigopoulos D., Drakoulis N. and Nicolaidou E.(2020). Impact of vitamin D receptor gene polymorphisms on vitiligo susceptibility and clinical features in a Southeastern European Caucasian population. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE.46: 1899-1907.
- Abdelmawla M., Abdullah S., Nasr M., Abd-Elghany Y. (2023). Evaluation of Vitamin D Receptor Gene Polymorphism in Vitiligo in Sharkia Governorate. The Egyptian Journal of Hospital Medicine. 90 (2): 2563-2566.
- Kriegel M.A., Manson J.E., Costenbader K.H., (2011). Does vitamin D affect risk of developing autoimmune disease?: a systematic review. Semin Arthritis Rheum. 40(6):512-531.e8.
- Li K., Shi Q., Yang L., Li X., Liu L., Wang L., et al., (2012). The association of vitamin D receptor gene polymorphisms and serum 25-hydroxyvitamin D levels with generalized vitiligo. Br J Dermatol.167(4):815-21.
- Koizumi H., Kaplan A., Shimizu T., Ohkawara A., (1997). 1,25-Dihydroxyvitamin D3 and a new analogue, 22-oxacalcitriol, modulate proliferation and interleukin-8 secretion of normal human keratinocytes. J Dermatol Sci 1997;15(3):207-13.
- Sauer B., Ruwisch L., Kleuser B., (2003). Antiapoptotic action of 1alpha,25-dihydroxyvitamin D3 in primary human melanocytes. Melanoma Res.13(4):339-47.
- Yalçin B., Sahin S., Bükülmez G., Karaduman A., Atakan N., Akan T., et al., (2001). Experience with calcipotriol as adjunctive treatment for vitiligo in patients who do not respond to PUVA alone: a preliminary study. J Am Acad Dermatol 44(4):634-7.
- Upala S., Sanguankeo A., (2016). Low 25-hydroxyvitamin D levels are associated with vitiligo: a systematic review and meta-analysis. Photodermatol Photoimmunol Photomed 32(4):181-90.
- Shakir. 2024. Association between IL-2 Levels and IL-2- rs2069762 Gene Polymorphism in Patients with Hashimoto’s Disease., Journal of Biomedicine and Biosensors., 4(1): 1 – 13. https://doi.org/10.5861 3/jbb411
- Kim , Kim S., Shin M., et al., (2022). Serum 25-Hydroxy Vitamin D Levels and Association of Vitamin D Receptor Gene Polymorphisms in Vitiligo. J Korean Med Sci. 2022 Apr 11;37(14):e110.
- Khurrum H., AlGhamdi K.M., (2016). The relationship between the serum level of vitamin D and Vitiligo: a controlled study on 300 subjects. J Cutan Med Surg 2016;20(2):139-45.
- Birlea S., Birlea M., Cimponeriu D., Apostol P., Cosgarea R., Gavrila L., Tigan S., Costin G. and Das P. (2006). Autoimmune diseases and vitamin Dreceptor Apa-I polymorphism are associated with vitiligo in a small inbred Romanian Community. Acta Derm Venereol. 86:209-14.
Research & Reviews : A Journal of Biotechnology
Volume | 14 |
Issue | 02 |
Received | May 22, 2024 |
Accepted | August 9, 2024 |
Published | August 12, 2024 |