Nanoyeast Supported on Silica Gel for The Continuous Flow Bioethanol Production

Year : 2024 | Volume :02 | Issue : 01 | Page : 1-7
By

Swara Pancholi,

Parth Naghera,

Manan Shah,

Indra Neel Pulidindi,

Suresh Selvaraj,

Prakash Vaithyanathan,

  1. M.Sc. Graduate, Department of Chemical Sciences, GSFC University, Vadodara,, Gujarat, India
  2. M.Sc. Graduate, Department of Chemical Sciences, GSFC University, Vadodara,, Gujarat, India
  3. M.Sc. Graduate, Department of Chemical Sciences, GSFC University, Vadodara,, Gujarat, India
  4. Scientific Consultant, Jesus’ Scientific Consultancy for Industrial and Academic Research (JSCIAR), Tharamani, Chennai, India
  5. Associate Professor, Department of Science and Humanities, ACE Engineering College, Ghatkesar, Secunderabad,, Telangana, India.
  6. Science Teacher and Innovator, 50, L.B road, Adyar, Chennai, India. [email protected]

Abstract

Nanomaterials outperform their bulk counterparts. Yet, production of nanomaterials, especially, nanoyeast is a challenge and is an upcoming field. Yeast is a fungal strain that consumes carbohydrates (glucose, sucrose, molasses and the like) and produces bioethanol, a potential transportation fuel. Impregnation methods are well known in catalysis to yield nanomaterials via better dispersion. An attempt has been made to modulate the size of yeast to increase the efficiency of carbohydrate conversion into bioethanol. Effective adsorbent, namely, silica gel, is developed to disperse small particles of yeast on the support. The supported biocatalyst (nanoyeast/silica gel) thus produced was packed in a fixed bed flow reactor. From a reservoir held vertically above the packed bed reactor, the carbohydrate feedstock (10 wt.%) was passed through the reactor. The reactor was packed with 50 g of the silica gel loaded with nanoparticles of yeast. In a very first attempt of its kind, successful conversion of sucrose to bioethanol is obtained at a modest flow rate of the feed. The product collected at the outlet after 24 hours of fermentation process in the fixed bed continuous flow reactor was characterized by 1H NMR to see the potential of the nanoyeast under modest loadings for the fermentation of carbohydrates. Indeed, the formation of bioethanol was observed in the 1H NMR spectrum (a 2 H, q at 1.029 ppm, and 3 H, t at 3.65 ppm) of the aliquot collected after 24 h of the fermentation of sucrose by the nanoyeast. This evidence is good enough to prove the formation of bioethanol from the metabolism of sucrose by the nanoyeast. However, in addition to peaks typical of bioethanol, the signals characteristic of unreacted sucrose were also seen. This shows that further optimization of the process is required. Thus a new avenue is opened to modulate the size of yeast particles using mild ultrasound irradiation (40 kHz) as a dispersing field and silica gel as dispersing medium. Much remains to be done in this direction pertaining to the characterization of the nanoyeast/silica gel biocatalyst. With the kind of synthetic strategy developed, even with no yeast particles visible to the naked eye, the supported catalyst could convert sucrose to bioethanol. It is, at the moment, surmised that the activity of the catalyst is attributable to the nanoyeast. However, detailed characterization of the size of the yeast particles with scanning electron microscopy is warranted, which will be a subject of our next communication.

Keywords: Bioethanol, continuous flow process, fermentation, glucose, nanoyeast, silica gel, support

[This article belongs to International Journal of Advance in Molecular Engineering (ijame)]

How to cite this article:
Swara Pancholi, Parth Naghera, Manan Shah, Indra Neel Pulidindi, Suresh Selvaraj, Prakash Vaithyanathan. Nanoyeast Supported on Silica Gel for The Continuous Flow Bioethanol Production. International Journal of Advance in Molecular Engineering. 2024; 02(01):1-7.
How to cite this URL:
Swara Pancholi, Parth Naghera, Manan Shah, Indra Neel Pulidindi, Suresh Selvaraj, Prakash Vaithyanathan. Nanoyeast Supported on Silica Gel for The Continuous Flow Bioethanol Production. International Journal of Advance in Molecular Engineering. 2024; 02(01):1-7. Available from: https://journals.stmjournals.com/ijame/article=2024/view=171132



References

1    Tabah B, Pulidindi IN, Chitturi VR, et al. Solar-energy-driven conversion of biomass to bioethanol: a sustainable approach, J Mat Chem A. 2017; 5: 15486-506p.
2    Tabah B, Varvak A, Pulidindi IN, et al.  Production of 1, 3-propanediol from glycerol via fermentation by Saccharomyces cerevisiae. Green Chem. 2016; 18: 4657-59p.
3    Klein M, Griess O, Pulidindi IN, et al.  Bioethanol production from Ficus religiosa leaves. J Environ Manag. 2016; 177: 20-5p.
4    Tabah B, Pulidindi IN, Chitturi VR, et al.  Utilization of solar energy for continuous production of bioethanol for energy applications. RSC Adv. 2016; 6: 24203-09p.
5    Kumar VB, Pulidindi IN, Kinel Y, et al.  Evaluation of the potential of Chlorella vulgaris for bioethanol production. Energy  Fuels. 2016; 30: 3161–6p.
6    Tabah B, Pulidindi IN, Chitturi VR, et al.  Solar energy driven simultaneous saccharification and fermentation (SSF) of starch to bioethanol for fuel cell applications. ChemSusChem. 2015: 8: 3497-503p.
7    Victor A, Pulidindi IN, Gedanken A. Assessment of Holocellulose for the production of Bioethanol by conserving Pinus radiata cones as renewable feedstock. J Environ manag. 2015; 162: 215-20p. 8    Korzen L, Pulidindi IN, Israel A, et al.  Marine integrated culture of carbohydrate rich Ulva rigida for enhanced production of bioethanol. RSC Adv. 2015; 59251-6p.
9    Tabah B, Pulidindi IN, Gedanken A.  A study on fermentation kinetics for accelerated production of bioethanol from glucose, sucrose, and molasses. J Bioprocess Biotech. 2015; 5: 232.
10  Korzen L, Pulidindi IN, Israel A, et al.  Single Step Production of Bioethanol from the Seaweed Ulva rigida using Sonication. RSC Adv. 2015; 5: 16223-9p.
11  Pulidindi IN, Kimchi BB, Gedanken A. Can cellulose be a sustainable feedstock for Bioethanol production? Renew energy. 2014; 71: 77-80.
12  Pulidindi IN, Gedanken A, Schwarz R, et al.  Mild sonication accelerates ethanol production by yeast fermentation. Energy Fuels. 2012; 26: 2352-6p.
13  West M,   Zurek N,  Hoenger A, et al. A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature.  J. Cell Biol. 2011; 193 (2):  333-46p.
14  Willaert R, Kasas S, Devreese B, et al.  Yeast Nanobiotechnology, Ferment. 2016; 2: 118p.
15  Rajesh D, Pulidindi IN, Pandurangan A, et al.  Pd-NiO decorated multiwalled carbon nanotubes supported on reduced graphene oxide as an efficient electrocatalyst for ethanol oxidation in alkaline medium. Appl Surf Sci. 2018; 442:787-96p.
16  Tangy A, Pulidindi IN, Gedanken A. Continuous flow biodiesel production from waste cooked oil using microwave irradiation and supported SrO catalyst. Bioresour. Technol. 2017; 224:333-41p. 17  Victor A, Pulidindi IN, Kim TH, et a;.Design of active solid acid catalyst for the optimization of glucose production   from Oryza Sativa straw. RSC Adv. 2016; 6:31-8p.
18  Kumar VB, Pulidindi IN, Gedanken A. Selective conversion of starch to glucose using carbon based solid acid catalyst. Renew Energy. 2015; 78: 141-5p.
19  Klein M, Varvak A, Segal E, et al. Sonochemical synthesis of HSiW/graphene catalysts for enhanced biomass hydrolysis. Green Chem. 2015. 17, 2418-25p.
20  Caylak B, Sukan FV. Comparison of different production processes for bioethanol. Turk. J. Chem. 1998; 22: 351-9p.


Regular Issue Subscription Original Research
Volume 02
Issue 01
Received August 16, 2024
Accepted August 21, 2024
Published September 9, 2024

Check Our other Platform for Workshops in the field of AI, Biotechnology & Nanotechnology.
Check Out Platform for Webinars in the field of AI, Biotech. & Nanotech.