Kanchan Kumari
- Student Department of Biotechnology, Patna University, Patna, Bihar India
Abstract
Berberine, a bioactive compound found in various plant species, exhibits diverse pharmacological properties with potential applications in pharmaceutical research. The biosynthesis of berberine involves several enzymatic steps, with (S)-tetrahydroprotoberberine oxidase playing a pivotal role. This study aimed to elucidate the structural and functional characteristics of (S)-tetrahydroprotoberberine oxidase to understand better its role in berberine biosynthesis and its potential biotechnological applications. Methods: Using bioinformatics tools and computational methods, the physicochemical properties, secondary structure, and molecular functions of the enzyme were analyzed. Predictions of solvent accessibility, transmembrane topology, and protein-protein interaction sites were made to gain insights into its structural organization and functional implications. Additionally, KEGG pathway analysis revealed the enzyme’s involvement in metabolic pathways relevant to berberine biosynthesis and plant metabolism. Results: The results provided valuable insights into the (S)-tetrahydroprotoberberine oxidase enzyme, highlighting its potential as a target for biotechnological applications and pharmaceutical development. However, the study acknowledges the limitations inherent in computational predictions and emphasises the need for experimental validation to confirm the findings. Conclusion: Overall, this research contributes to our understanding of (S)-tetrahydroprotoberberine oxidase and lays the groundwork for future studies aimed at exploring its biochemical properties and applications in various fields. Overall, this research contributes to our understanding of (S)-tetrahydroprotoberberine oxidase and lays the groundwork for future studies aimed at exploring its biochemical properties and applications in various fields. Future work should focus on experimental validation of the computational predictions, which will be crucial for harnessing the enzyme’s full potential in pharmaceutical and biotechnological innovations.
Keywords: Berberine, (S)-tetrahydroprotoberberine oxidase, THP Oxidase, Berberis wilsoniae, InSilico Analysis
References
Adejoke HT, Louis H, Amusan OO, Apebende G. A Review on Classes, Extraction, Purification and Pharmaceutical Importance of Plants Alkaloid. 2019 Jan; 2:130-139.
Ali F, Alom S, Zaman MK. (2021). Berberine: A Comprehensive Review on its Isolation, Biosynthesis, Chemistry and Pharmacology. 2021 Oct;33(11):2548-2560.
Ali F, Alom S, Zaman MK. Berberine: A Comprehensive Review on its Isolation, Biosynthesis, Chemistry and Pharmacology. 2021 Oct;33(11):2548-2560.
Amann M, Nagakura N, Zenk MH. Purification and properties of (S)-tetrahydroprotoberberine oxidase from suspension-cultured cells of Berberis wilsoniae. 1988 Jul;175(1):17-25.
Cheng Z, Kang C, Che S, SU J, Sun Q, Ge T, Guo Y, Lv J, Sun Z, Yang W, Li X, Cui R. Berberine: A Promising Treatment for Neurodegenerative Diseases. 2022 May;13:845591.
Debnath B, Singh WS, Das M, Goswami S, Singh MK, Maiti D, Manna K. Role of plant alkaloids on human health: A review of biological activities. 2018 Sep;9:56-72.
Deng X, Zhao I, Fang T, Xiong Y, Ogutu C, Yang D, Vimolmangkang S, Liu Y, Han Y. Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus. 2018 Jun;5:29.
Dey P, Kundu A, Kumar A, Gupta, M, Lee BM, Bhakta T, Dash S, Kim HS. Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids). 2020 Mar:505-567.
Dincheva I, Badjakov I, Galunska B. New Insights into the Research of Bioactive Compounds from Plant Origins with Nutraceutical and Pharmaceutical Potential. 2023 Jan;12(2):258.
Heinrich M, Mah J, Amirkia V. Alkaloids Used as Medicines: Structural Phytochemistry Meets Biodiversity – An Update and Forward Look. 2021 Mar;26(7):1836.
Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. 2018 Jan;7(1):1-7.
Majolo F, Delwing LKDOB, Marmitt DJ, Bustamante-Filho IC, Goettert MI. Medicinal plants and bioactive natural compounds for cancer treatment: Important advances for drug discovery. 2019 Jun;31:196-207.
Martirosyan D, Miller E. (2018). Bioactive Compounds: The Key to Functional Foods. 2018 Jul;8(7):36-39.
Mbuni YM, Wang S, Mwangi BN, Mbari NJ, Musili PM, Walter NO, Hu G, Zhou Y, Wang Q. Medicinal Plants and Their Traditional Uses in Local Communities around Cherangani Hills, Western Kenya. 2020 Mar;9(3):331.
Mujtaba Md A, Akhter, Habban M, Sarfaraz M, Ali, Daud, M, Hussain, Afzal. An Updated Review on Therapeutic Potential and Recent Advances in Drug Delivery of Berberine: Current Status and Future Prospect. 2022 Feb;23(1):60-71.
Nasim N, Sandeep IS, Mohanty S. Plant-derived natural products for drug discovery: current approaches and prospects. 2022 Oct;65(3):399-411.
Pandaressh MD, Mythri RB, Bharath MMS. Bioavailability of dietary polyphenols: Factors contributing to their clinical application in CNS diseases. 2015 Jul:1-11.
Pereira AG, Cassani L, Garcia-Oliveira P, Otero P, Mansoor SS, Echave J, Xiao J, Simal-Gandara J, Prieto MA. Plant alkaloids: Production, extraction, and Potential Therapeutic properties. 2023 Feb:157-200.
Prajwala B, Raghu N, Gopenath TS, Kaginelli SB, Karthikeyan M, Ashok G, Ranjith MS, Srinivasan V, Basalingappa KM. Berberine And Its Pharmacology Potential: A Review. 2020 Nov;7(5):115-123.
Salmeron-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. Worldwide Research Trends on Medicinal Plants. 2020 May;17(10):3376.
Santhan P. A field study on Indian medicinal plants. 2020 Aug;8(4):198-205.
Santos JSD, Cirino JPG, Carvalho PDO, Ortega MM. The Pharmacological Action of Kaemferol in Central Nervous System Diseases: A Review. 2020 Jan;11:565700.
Shakya AK. Medicinal plants: Future source of new drugs. 2016 Jan;4(4):59-64.
Sobhani Z, Akaberi M, Amiri MS, Ramezani M, Emami SA, Sahebkar A. Medicinal Species of the Genus Berberis: A review of Their Traditional and Ethnomedicinal Uses, Phytochemistry and Pharmacology. 2021:547-577.
Yudharaj P, Shankar M, Sowjanya R, Sireesha B, Naik EA, Priyadarshini RJ. Importance and uses of medicinal plants – An Overview. 2016 Jan;7(2):67-73.
| Volume | |
| Received | June 22, 2024 |
| Accepted | June 29, 2024 |
| Published | July 1, 2024 |