Phytochemical Screening and Biological Activity of Pomegranate Extracts

Open Access

Year : 2025 | Volume : 12 | Issue : 02 | Page : –
    By

    B. Premkumar,

  • K. Vinothkumar,

  • V. Mugilan,

  • P. Nandhini,

  • P. Venkatesan,

  1. Professor & HOD, Department of Pharmaceutics & Biotechnology, Sree Abirami College of Pharmacy, Eachanari, Coimbatore, Tamil Nadu, India
  2. Student, Department of Pharmaceutics & Biotechnology, Sree Abirami College of Pharmacy, Eachanari, Coimbatore, Tamil Nadu,
  3. Student, Department of Pharmaceutics & Biotechnology, Sree Abirami College of Pharmacy, Eachanari, Coimbatore, Tamil Nadu,
  4. Student, Department of Pharmaceutics & Biotechnology, Sree Abirami College of Pharmacy, Eachanari, Coimbatore, Tamil Nadu, India
  5. Student, Department of Pharmaceutics & Biotechnology, Sree Abirami College of Pharmacy, Eachanari, Coimbatore, Tamil Nadu, India

Abstract

Pomegranates are renowned for their abundance of bioactive compounds that exhibit potent antioxidant, anti-inflammatory, and antimicrobial properties, making them a subject of growing interest in natural medicine. This study aimed to compare the biological activities of ethanolic extracts derived from different parts of the pomegranate plant—specifically, the bark, peel, and leaves. A comprehensive investigation was conducted to evaluate their phytochemical composition and assess their antimicrobial, antioxidant, and anticancer properties. The antimicrobial activity of each extract was tested against Staphylococcus aureus, Klebsiella pneumoniae, and Aspergillus niger using the Kirby-Bauer well diffusion method, with zones of inhibition measured to determine efficacy. Antioxidant capacity was evaluated through total antioxidant activity, hydrogen peroxide (H₂O₂) scavenging assays, and Ferric Reducing Antioxidant Power (FRAP) assays. Antitumor study was assessed using the MTT assay. Among the three extracts, the bark extract demonstrated the most significant biological activity and was subsequently chosen for advanced anticancer studies on liver cell lines, where it showed marked cytotoxic effects. These findings suggest that pomegranate bark holds considerable promise as a source of therapeutic agents, particularly in the context of cancer treatment. Continued research may pave the way for the development of novel health-promoting formulations derived from pomegranate.

Keywords: Pomegranate, phytochemical, Kirby-Bauer well diffusion, antioxidant, antimicrobial, MTT assay.

[This article belongs to Research & Reviews: A Journal of Pharmacognosy ]

How to cite this article:
B. Premkumar, K. Vinothkumar, V. Mugilan, P. Nandhini, P. Venkatesan. Phytochemical Screening and Biological Activity of Pomegranate Extracts. Research & Reviews: A Journal of Pharmacognosy. 2025; 12(02):-.
How to cite this URL:
B. Premkumar, K. Vinothkumar, V. Mugilan, P. Nandhini, P. Venkatesan. Phytochemical Screening and Biological Activity of Pomegranate Extracts. Research & Reviews: A Journal of Pharmacognosy. 2025; 12(02):-. Available from: https://journals.stmjournals.com/rrjopc/article=2025/view=215695


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Regular Issue Open Access Original Research
Volume 12
Issue 02
Received 12/06/2025
Accepted 25/06/2025
Published 03/07/2025
Publication Time 21 Days
Total Visits: 44


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